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Next-Generation Sequencing Evaluating DNA Yields

Analyzing the blood of patients with myelodysplastic syndrome (MDS) for the cell-free DNA using the NGS (next generation sequencing) does yield more accurate results as opposed to the current approach of the Sanger sequencing method. Detecting the abnormality in the gene that is responsible for causing the disorder is done by analyzing the DNA that is cell-free versus the DNA received from the patients’ blood cells. This information can be found in the published study on Genetic Testing and Molecular Biomarkers which is a peer reviewed paper from Mary Ann Liebert, Inc., publishers.

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Maher Albitar, Ferras Albitar, and coauthors from the NeoGenomics Laboratories, Irvine, CA investigated the three techniques that are used to associate DNA mutations with MDA which is a group of cancers that effects the bone marrow. If the molecular abnormalities are accurately analyzed in the patients with MDS early diagnosis is then possible and helps guide in patient care. The cellular DNA and cell-free DNA from the cancerous cell that is found in the bone marrow will be shed into the patients’ blood.
The Next-generation sequencing is obtainable for the assessment of genomic alterations in the non-small-cell lungs cancer which is referred to as NSCLC. The performance and the clinical utility is not well categorized is a concern however the sequence has been known to sequence many cancer-associated genes.

General Information About Myelodysplastic Syndromes

A Myelodysplastic syndrome is known as a group of cancers that form in the bone marrow from immature blood cells which do not become healthy or mature into blood cells. There are different types of blood cells that are diagnosed which are based on changes in the bone marrow and blood cells.

Myelodysplastic Syndromes

The treatment of radiation or chemotherapy may affect the chance of picking up myelodysplastic syndrome. The most important signs or symptoms of MDS is feeling tired and the shortness of breath. There are certain tests that can be conducted to detect and diagnose MDS however certain factors do affect the prognosis as well as the treatment options.

The anatomies of the bones are made up of spongy bone, compact bone, and the bone marrow. The compact bone is found on the outer layer while the spongy bone is mostly found on at the ends of each bone and this contains the red marrow. Found at the center of most bones is bone marrow that contains many blood vessels. The two types of bone marrow that can be found is red marrow and yellow marrow. The red marrow comprises of the blood stem cells that will become red blood cells, platelets, or white blood cells. The yellow marrow is made mostly of fat.

The blood stem cells may become a myeloid stem cell or a lymphoid stem cell which will become a white blood cell. The myeloid stem cells may become one of three types of the mature blood cells being; red blood cells that will carry oxygen as well as other substances to the tissues in the body, platelets that will form blood clothes if the person is bleeding or white blood cell that will fight off infectious diseases.